Properties of the A-to-I editing sites in F. graminearum. (A) The number of each type of RNA variant (gDNA → cDNA) sites per million mapped unduplicated reads identified in the two RNA-seq data of 8-dpf perithecia. (B) Histogram and box plot showing the frequency of RNA editing levels. The majority of editing sites have editing levels <30%. (C) The percentage of marked categories of genes that have editing sites of different editing levels. Genes that were specifically expressed (Perithecium-specific) or up-regulated (Perithecium-up) in perithecia were identified by comparative analysis of RNA-seq data of conidia, hyphae, and 8-dpf perithecia (see Supplemental Methods). (D) The distribution of 26,056 A-to-I editing sites. Because only a few genes have known UTRs in F. graminearum, we used the 500-bp region upstream of the start codon and the 500-bp region downstream from the stop codon to represent the 5′- and 3′-UTRs, respectively.
