The global pattern of variation in N. tetrasperma. (A) The phylogenetic relationships of all N. tetrasperma strains used in this study, inferred from 2,259,433 variable sites on the autosomes. A subtree excluding N. discreta, N. crassa, and N. hispaniola is shown. Numbers on the branches indicate the bootstrap support for that relationship expressed as a proportion. (B) Principal component analysis (PCA) of genetic variation (509,199 biallelic autosomal SNPs) across the global sample of N. tetrasperma strains. The first two principal components are shown. (C) Population structure of N. tetrasperma inferred from 9000 SNPs (1500 from each of the six autosomes) using InStruct at K = 6. Lineages color coded in A, B, and C according to the legend in B. (LA) Louisiana; (NZ) New Zealand; (UK) United Kingdom; (HI) Hawaii; (MX) Mexico; (LB) Liberia.
