Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq

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Figure 5.
Figure 5.

Analysis of NGS-validated and NGS-invalidated off-target sites. Plots of relative indel frequencies (log scale) at off-target sites harboring the number of mismatches indicated in the entire 20-nt sequence (A) or the 10-nt seed sequence (B,C). NGS-tested sites (A) were divided into two groups: validated sites (B) and invalidated sites (C). NGS-validated sites and NGS-invalidated sites were those with indel frequencies above and below, respectively, noise indel levels.

This Article

  1. Published in Advance January 19, 2016, doi: 10.1101/gr.199588.115 Genome Res. 26: 406-415

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