Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq

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Figure 4.
Figure 4.

Indel frequencies determined using targeted deep sequencing at off-target sites. (A) Schematic of conventional sgRNAs (GX19 sgRNA) and modified sgRNAs (ggX20 sgRNA). Indel frequencies at NGS-validated on- and off-target sites for the EMX1 (B), HEK293-3 (C), and RNF2 sgRNAs (D). Specificity ratios calculated by dividing the indel frequency at the on-target site with that at an off-target site for the EMX1 (E), HEK293-3 (F), and RNF2 sgRNAs (G).

This Article

  1. Published in Advance January 19, 2016, doi: 10.1101/gr.199588.115 Genome Res. 26: 406-415

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