Figure 1.
Improving Digenome-seq analysis. (A) Genome-wide Circos plots of in vitro DNA cleavage scores. Human genomic DNA (red) or RGEN-digested genomic DNA (green) was subjected to whole-genome sequencing. The few false-positive sites found in intact genomic DNA are not visible because their cleavage scores are not high. (B) Schematic overview of Digenome-seq using sgRNA transcribed from an oligonucleotide duplex or a plasmid. (C) Sequence logos of cleavage sites obtained using sgRNA transcribed from an oligonucleotide duplex or a plasmid.
