Experimental validation for T-ALL Jurkat cell circuitry. (A) Core regulatory circuit containing GATA3, MYB, RUNX1, and TAL1 for T-ALL Jurkat cells. (B) ChIP-seq data for H3K27ac, MYB, RUNX1, TAL1, and GATA3 showing binding of the TF to each of the SEs in the T-ALL Jurkat cell core circuit. SE genomic locations are depicted by red lines on top of the tracks. (C) Boxplots showing fold change (FC) in expression for Jurkat cells transfected with the indicated shRNAs vs. control shRNAs, for either the set of candidate core TFs displayed in A (red) or the full set of TFs considered expressed in Jurkat cells (blue). P-values quantifying the difference between the two sets were calculated using a Wilcoxon test.
