H1 ESC core and extended regulatory circuitry. (A) (Left) CRC model for H1 human embryonic stem cells. The role of each TF in ESC pluripotency and self-renewal is listed in Table 1. (Right) H1 hESC extended regulatory circuit. Examples of SE-assigned genes that are predicted to be bound by each of the TFs in the CRC. The role of these factors in ESC pluripotency and self-renewal is listed in Supplemental Table S5. (B) ChIP-seq data for H3K27ac, POU5F1, SOX2, and NANOG showing binding of the TFs to each of the SEs of the SE-assigned TFs in the hESC CRC. SE genomic locations are depicted by red lines on top of the tracks. (C) Pie charts showing the percentages of SE-assigned genes (top row) or all expressed genes (bottom row) whose regulatory sequences are predicted to be bound by increasing numbers of hESC candidate core TFs. (D) Diagram showing putative transcriptional regulation of miR-371a on SOX2 expression in hESCs.
