Quantitative BrdU immunoprecipitation method demonstrates that Fkh1 and Fkh2 are rate-limiting activators of replication origins that reprogram replication timing in G1 phase

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Figure 6.
Figure 6.

Fkh1 stimulates origin firing in subtelomeric heterochromatin. Strains OAy1071 (fkh1Δ fkh2Δ GAL-FKH1) and OAy1073 (fkh1Δ fkh2Δ) were induced and released into HU as described in Figure 2A and subjected to qBrdU-seq analysis. (A) Data are plotted for Chromosome XI. (B) Averaged BrdU signal for subtelomeric origins detected to fire. (C) Data are plotted for Chromosome V, highlighting the subtelomeric ars501Δ::ARS305 locus.

This Article

  1. Published in Advance January 4, 2016, doi: 10.1101/gr.196857.115 Genome Res. 26: 365-375

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