Quantitative BrdU immunoprecipitation method demonstrates that Fkh1 and Fkh2 are rate-limiting activators of replication origins that reprogram replication timing in G1 phase

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Figure 5.
Figure 5.

Fkh1-OE increases Fkh1 occupancy at stimulated origins. Strains JPy105 (WT, Fkh1-Myc) and JPy106 (Fkh1-Myc-OE) arrested and induced (but not released into S phase) as described in Figure 2A were subjected to ChIP-chip analysis of Fkh1. (A) ChIP signal along Chromosome XI is plotted with origins indicated above. (B) The average ChIP signal is plotted for 5-kb regions aligned on origins of the indicated groups. (C) Box-and-whisker plots of the distributions of ChIP signals for individual origins within the indicated origin groups.

This Article

  1. Published in Advance January 4, 2016, doi: 10.1101/gr.196857.115 Genome Res. 26: 365-375

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