Concerted activities of Mcm4, Sld3, and Dbf4 in control of origin activation and DNA replication fork progression

Replication profiles and checkpoint response in Mcm4 NSD mutants. (A,B) Yeast cells were synchronized in G1 phase and released into YPD containing 0.05% MMS and 0.5 mM EdU for 50 min. (A) Replication profiles of Chromosome IV for the wild type and the indicated Mcm4 NSD mutants. (B) Distribution of fork progression from origins shown as individual width–height plots and box graph, which excludes peaks with heights <30% of the maximal height scale. (C) Cells from the indicated strains were synchronized in G1, released into YPD containing 0.05% MMS, and collected at indicated time points. Protein samples were prepared using TCA extraction and analyzed by immunoblot.