Figure 7.
Checkpoint-resistant mutations of SLD3 and DBF4 do not affect timing of late origin firing in an unperturbed S phase. Yeast cells were synchronized in G1 phase and released into YPD containing 0.5 mM EdU for 25 min. (A) Replication profiles of Chromosome IV for WT, single, double, and triple mutants with mcm4Δ74-174, sld3-38A, and/or dbf4-19A alleles as indicated on the top left of each profile. (B) Plots comparing the peak height between duplicate samples of mutant strains (red and orange) to a wild-type reference (blue). Data also presented in Supplemental Figure S3. Peak heights for each sample were scaled so that the 90% quantile is equal to 1.0.
