Figure 5.
Late origin firing in HU in the absence of checkpoint signaling kinase. Yeast cells were synchronized in G1 phase and released into YPD containing 0.2 M HU and 0.5 mM EdU for 90 min. (A) Replication profiles of Chromosome IV for WT, sml1Δ, mec1Δ sml1Δ, rad53Δ sml1Δ, and the mcm4Δ74-174 sld3-38A dbf4-19A triple mutant cells. (B) Distribution of fork progression from origins shown as individual width–height plots and box graph, excluding peaks with heights <30% of the maximal height scale.
