DNA methylation is required for photoperiodic-mediated diapause response. (A) The progeny of control females (GFP dsRNAi-injected) exhibit the normal photoperiodic response typical of Nasonia: low diapause incidence induced in long day (LD: 18-h light, white box) compared with short day (SD: 6-h light, gray box), as tested after 5 d at 18°C. (***) P < 0.01. In females injected with dsRNAi targeting Dnmt1a (B) and Dnmt3 (C), the difference in diapause levels between long and short day is abolished (18–20 females in each group, progeny of each female collected from two hosts, average n = 24 larvae per female). The line inside each box represents the median; the top and the bottom represent the 75th and 25th percentiles, respectively. (D) Nasonia developing pupae (yellow) and diapausing larvae (dark).
