Positioning information is dispersed across mononucleosomal DNA. (A) MNase end-labeling analysis of wild-type (WT) and the dyad, linker, and internal mutant strains. Ovals represent nucleosomes across the ura4 gene. Colored sections indicate the third of the mononucleosomal DNA sequence that was replaced by synonymous codons in the dyad (green), linker (orange), or internal (black) regions of nucleosomes 1–6. The restriction site for HindIII and the hybridization probe are indicated. (B) Nucleosome occupancy maps of the four strains and DANPOS analysis of nucleosome positioning of the three mutant strains relative to the wild type. Symbols are as in Figure 1.
