Mononucleosomal DNA encodes portable positioning information. (A) White ovals represent nucleosomes across the ura4 region as shown in Figure 1, A and C. Colored ovals 2–5 indicate regions that were replaced by nonadjacent mononucleosomal sequences from unrelated genomic regions. In nucleosomes 1 and 6, only colored regions were replaced by equivalent regions from other mononucleosomal DNA sequences. The restriction site for PsuI (P) and the hybridization probe (green) are indicated. (B) The MNase end-labeling analysis of wild-type cells (WT) and of the chimeric construct generated a comparable positioning pattern. Nucleosome positioning was lost after individual randomization of the sequences underlying the colored regions in A (Random).
