Figure 2.
Sequence changes destabilize nucleosome positioning in nontranscribed regions. (A) MNase end-labeling analysis from wild-type cells (WT) and from the strain harboring randomized sequences underlying nucleosomes 3 and 4 (green). (B) (Top) The position of the SPAC6F6.11c gene and the kanMX marker gene is indicated. Restriction sites for NsiI (N) and the position of the hybridization probe are indicated. (Middle) Nucleosome occupancy map of the wild-type strain with nucleosomes numbered as in A. (Bottom) DANPOS analysis of nucleosome positioning of the Random strain relative to the wild type. Vertical dashed lines indicate the position of nucleosomes 3 and 4. Other symbols are as in Figure 1.
