Figure 1.
Sponge design in pLCE lentiviral transfer vector. The miR-128 sponge sequence is comprised of six tandem repeats of sequence complementary to miR-128. The sequence is designed with a mismatch at nucleotides 9–12 and each repeat is spaced by a 6- to 8-nt sequence. The sponge is driven by a CMV promoter downstream from a reporter eGFP gene. miRNA binding sequence is AAAGAGACCAACCACTGTGA.
