The activity of both the ATPase and RING domain of Rad16 determine its chromatin occupancy before and after UV irradiation. (A) Representation of the linear structure of Rad16. The amino acids targeted by the point mutations introduced in the ATPase (K216A) and RING domains (C552A, H554A) are highlighted. (B–E) Composite plots of Rad16 chromatin occupancy in the mutants described. Mutated Rad16 binding data around Abf1 binding sites and ORF structures in the absence of UV irradiation (dark blue) and 15 min after UV irradiation (green) are shown here. The Rad16 RING mutant binding data (dashed lines) are shown in B (around Abf1 binding sites) and C (around ORFs). The binding data for Rad16 ATPase domain mutant (dotted lines) are shown in D (around Abf1 binding sites) and E (around ORFs). Lines show the means of three data sets per condition, and shaded areas show the SEM.
