Figure 1.
The circular synIXR synthetic chromosome comprises 43 segments, numbered consecutively as shown, with loxPsym sites (orange bars) serving as junctions between adjacent segments. Arrows indicate genes, with colors denoting essential genes (red), auxotrophic markers (purple), other nonessential genes (light blue), and the chloramphenicol resistance marker (green). The centromere, in segment 2, is shown as a black circle. The left and right ends of each segment are denoted “L” and “R,” and loxPsym sites are denoted by the unique half-sites of the segments they join. For example, the loxPsym site at the junction between segments 1 and 2 involves half-sites 1R and 2L.
