Figure 4.
Interplay between sequencing depth, LM-PCR depth, and RNA fusions. (A) Total number of fusions observed in each sample plotted against the sequencing depth. (B) The number of fragments in six tumors using the shLM-PCR technique, ordered in increasing number of fragments which is a semiquantitative indication of clonality and the number of fusions which support these shLM-PCR junction fragments. (C) Relationship between shLM-PCR number of supporting fragments and RNA fusion support. As more supporting shear fragments are observed, the percentage of insertions with observed fusions increases.
