Genome-wide analysis of translational efficiency reveals distinct but overlapping functions of yeast DEAD-box RNA helicases Ded1 and eIF4A

A significant number of genes require both Ded1 and eIF4A for WT translation. (A) Scatterplot of TE of tif1-ts versus WT cells at 37°C, with 68 genes showing a ≥twofold change in the mutant at FDR < 0.01, colored red. (B) Venn diagram of genes exhibiting ≥twofold reductions in tif1-ts (at 37°C) and ded1-cs strains. (C) Smooth scatterplot of the indicated log₂ ΔTE values, generated as in Figure 1E. (D) Hierarchical clustering analysis of absolute ΔTE values for 4696 expressed genes from the indicated mutant versus WT comparisons (excluding genes with >64-fold changes in TE) as a heat map using the R heatmap.2 function from the R “gplots” library, using the default hclust hierarchical clustering algorithm.