3′ splice sites in dissected gonad samples that deviate from the canonical AG dinucleotide. 32P RT-PCR and RNA sequencing coverage tracks of handpicked glp-4 whole worm, wild-type whole worm, and wild-type germline RNA showing tissue-specific expression of isoforms with alternative 3′ splice sites. Analysis of par-4 (A), ubxn-6 (B), and icd-2 (C) RNA sequencing reveals significant usage of noncanonical 3′ splice sites primarily in germline-containing samples. Sequences above each set of coverage tracks show the dinucleotide set found at the somatic site preceded by the dinucleotide set found at the germline site (underlined) and the accompanying sequence immediately upstream. Genomic and mRNA sequences in these regions were validated.
