Figure 1.
Developmental and tissue-specific changes in alternative splicing of the top-1 gene. (A) Exon inclusion of top-1 alternative cassette exon occurs with the onset of germline development. A 1% agarose gel of reverse-transcription-polymerase chain reaction (RT-PCR) products of RNA extracted from handpicked C. elegans whole worms at indicated developmental stages. Exon inclusion and skipping products are indicated at right. (B) A 2100 Bioanalyzer image of top-1 RT-PCR products of RNA extracted from L4+1 day C. elegans dissected wild-type gonads, adult whole worms, and dissected heads, along with glp-4(bn2ts) mutant whole worms that fail to develop a germline.
