Figure 5.
Heritable chromosomal deletions induced by CRISPR/Cas9. (A) Summary of detected large deletions between two target sites. Large deletions were identified in man2a1, mgat1b, man2b1, and mgat3a. (B) Graphical representation of the man2a1 locus and the sequence of the deletion interval. (C–E) Sequence of the deleted interval for mgat1b, man2b1, and mgat3a. The target sites are marked in gray, while the PAM sites are underlined. The sequences were obtained from the PCR products across the two target sites. The top strand represents the wild-type sequence and the bottom strand represents the junction sequences between two target sites. The size of the deletion is indicated on the right side.
