CELF1 binds to 3′ UTRs and regulates message stability in a dose-dependent fashion. (A) Mean CELF1 CLIP density at positions along 3′ UTRs in heart, muscle, and myoblasts. (B) Mean conservation in sets of 3′ UTRs with and without CELF1 CLIP clusters with similar expression levels and UTR lengths (shading represents SEM). (C) Expression of Clcn1 in muscle based on RNA-seq (mean ± SD) at various times following CELF1 overexpression (bottom); CLIP density in Clcn1 3′ UTR (top). (D) Mean log expression change following CELF1 induction (7 d over control) for transcripts grouped by number of CELF1 CLIP clusters in their 3′ UTRs. Transcripts with greater CLIP cluster density are down-regulated more strongly in heart and muscle (number of genes in each category listed above). Significance was assessed by rank-sum test, where each CLIP cluster bin was compared to the zero CLIP cluster bin. See also Supplemental Table S6.
