Genomic redistribution of GR monomers and dimers mediates transcriptional response to exogenous glucocorticoid in vivo

GR^dim partitions the GR cistrome in primary macrophages. (A) Top-ranked de novo motifs from HOMER for the WT-selective and common GR binding sites from ChIP-seq in primary macrophages. Comprehensive motif results are reported in the Supplemental Material. (B) Box plots interrogating the co-occupancy of macrophage TFs at GR binding sites. (C) Regions named after the nearest gene that include WT-selective and common sites were assayed by a luciferase reporter in the absence (−) or presence (+) of dexamethasone (Dex) and cotransfection of empty vector (E.V.), GR, GR^dim, or the DNA-binding mutant GR^C421G. (D) Response of WT-regulated genes (up or down) from LPS- and dex-treated macrophages in GR^dim-derived macrophages under the same treatment.