Characterizing and controlling intrinsic biases of lambda exonuclease in nascent strand sequencing reveals phasing between nucleosomes and G-quadruplex motifs around a subset of human replication origins

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Figure 1.
Figure 1.

The MYC G-quadruplex (G4) impedes λ-exo digestion. (A) Diagram of the plasmid digestion experiment. The predicted sizes of single-stranded fragments resulting from the inability of λ-exo to digest through the G4 motifs are shown. (B) Digestion of 3′-labeled BglII linearized pFRT.myc6xERE in glycine-KOH (pH 9.4). Double-stranded and single-stranded DNA were used as indicated. (C) Digestion of single-stranded DNA from pFRT.myc6xERE and deletion mutants of Pu30 and Pu27 in four different buffers. The pH of the buffer and the base used to titrate the pH are indicated.

This Article

  1. Published in Advance February 18, 2015, doi: 10.1101/gr.183848.114 Genome Res. 25: 725-735

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