The dynamics of gene regulation over 24 h of capsule induction. (A) Heat map of genome-wide expression profiling: (blue) low expression; (yellow) high expression. Examples of functional annotations of genes in each cluster are indicated. (B) Regulatory relations among groups of capsule-involved genes clustered by temporal expression pattern: (circles) groups of regulators clustered by their temporal expression patterns (insets); text denotes representative functions of their activated (green) or repressed (red) targets; (boxes) capsule-involved target genes clustered by their temporal expression patterns (insets); text provides examples of target gene functions; (green arrows) activation of designated targets; (red T-heads) repression of designated targets. Regulator Group 1 is Clr1, Hap5, Nrg1, Pkr1, and Ssn801; Regulator Group 2 is Cac1, Cep3, Cir1, Clr2, Fap1, Fhl1, Fkh2, Gat1, Mcm1, Pdr802, Sp1, Swi6, and Usv101; Regulator Group 3 is Clr6, Hog1, Mbs1, Ste12alpha, and Tup1; Regulator Group 4 is Ada2, Clr3, Clr4, Clr5, Ecm2201, Fkh101, Gat201, Hap3, and Rim101. (NSS) Nucleotide sugar synthesis; (NST) nucleotide sugar transport; (ROS) reactive oxygen species.
