(A) Iqub whole mount (WM, left) mid-sagittal section showing staining of ventricular ependyma throughout the brain including the lateral and fourth ventricles (a), as well as staining of the glomeruli in the olfactory bulb (b), and coronal frozen section (FS, right) staining of forebrain illustrating ependymal staining of the lateral ventricle (c). (B) Amer2 FS retina with intense staining of the photoreceptor cell layer (a) and diffuse scattered staining of the inner nuclear layer (b). (C, left) Col6a2 aorta staining in WM showing distinct staining of the aortic arch and the carotid arteries (a) with no staining of white adipose tissue (b) or the left ventricle (c). (Right) FS of the aorta shows smooth muscle only staining for LacZ (d). (D, left) Ptma WM lung staining showing a variegated staining pattern. (Right) This uneven, variegated pattern of staining is also found in FS with individual cells staining in the parenchyma (a), as well as nonciliated cells in the bronchial epithelium (b). (E, left) Nudt19 liver staining is shown for WM, indicating that the bile ducts (a) and gall bladder (b) stain for LacZ. (Right) FS staining shows that epithelial cells in the ducts are expressing the lacZ reporter. (F, left) Aard pancreas staining by the WM method shows a distributed punctate staining pattern suggestive of islets. (Right) Aard FS confirms the inference of islet staining from the WM studies and further shows that a subset of cells stains in the islet (b), while ducts (c) and exocrine tissue (d) do not stain in Aard. (G, left) Spp2 kidney staining by WM shows intense staining of the tubules in the cortex only, with no staining in the medulla or hilus. (Right) Spp2 FS shows renal cortical staining in tubules (a), Bowman's capsule (b), and some cells/membranes in the interior of Bowman's capsule (c). (H, left) Jazf1 WM testis staining shows the characteristic WT nonspecific pattern of staining in the epididymis (a) with a 2-mm-wide band of minimal staining separating caput LacZ and additional LacZ staining in the body of the epididymis. Although faint, there is above-background WM staining in the seminiferous tubules (b) within the intact tunica, while the associated adipose tissue (c) does not stain. (Right) FS staining of Jazf1 shows intense staining of seminiferous tubule lumen (d), suggesting that immature sperm express the lacZ reporter. Some, but not all spermatogonia also appear to stain in the FS (e). (I, left) Ccl9 intestinal staining by WM shows a uniform punctate pattern of staining in the wall of the small intestine (a). The vasculature is clearly demarked on the surface of the intestine since it does not stain. Peyer's patches (b) also do not stain, except for a small band of staining encircling each dome in the patch (b). (Right) FS staining of Ccl9 shows staining primarily in the Crypts of Lieberkuhn (c), but there are some cells in the microvilli also staining for LacZ (d).
