Enhancer elements are maintained in cohesin-deficient thymocytes. The Cd8 (A) and the Ppp1r16b region (B) illustrate the maintenance of H3K27ac in cohesin-deficient thymocytes. Binding of the cohesin subunits RAD21 and SMC1A as well as CTCF is shown for reference (see below). (C) Rad21 deletion does not abolish H3K27ac marking of conventional enhancers (left), newly established developmentally regulated enhancers (red, middle), or super-enhancers (right). (D) Enhancers and super-enhancers were stratified according to the ratio of H3K27ac in Rad21-deficient thymocytes over wild type. The frequency of deregulated genes is shown for the top, middle, and lower third of enhancers (left) and super-enhancers (right).
