Association of subclonal fraction with TP53 mutation and event-free survival. (A) Clonal (yellow) and subclonal (blue) mutation frequencies and their association with G-CIMP methylation phenotype (blue) and p53 pathway-related alterations. The total number of sSNVs per patient is indicated in the top panel. (B) Patients with non-G-CIMP GBM harboring a higher subclonal mutation frequency (top 30% of non-G-CIMPs; Group II; blue) exhibited a longer event-free survival time compared to non-G-CIMP patients, whose tumor cell mutations had higher clonal frequency (bottom 30% of non-G-CIMPs; Group III; red). G-CIMP cases are grouped separately into Group I (dark green) and show event-free survival similar to that of Group II. Only patients younger than 55 yr of age were included in Groups II and III, and all G-CIMP cases were included in Group I.
