Inference of mutational clonality and association with patient age. (A) Validation of clonal and subclonal classifications using multisector sequencing. Mutations found in all nonoverlapping tumor sectors from the same tumor were defined as ubiquitous mutations, otherwise as private. Ubiquitous and private mutations were further subdivided into clonal and subclonal mutations, resulting in four categories (ubiquitous/clonal, ubiquitous/subclonal, private/clonal, and private/subclonal). (B) The clonal mutation frequency correlates with age across 221 primary GBMs with available age information (left panel), whereas the subclonal mutation frequency does not (right panel). The patients were separated into discrete age groups by intervals of 10 yr.
