Figure 3.
UBTF1/2 binds histone genes. (A–C) IGV (Integrated Genome Viewer) screenshots of mapped reads from UBTF1/2 ChIP and input gDNA at mouse histone gene cluster 1 (A), cluster 2 (B), and histone variant genes H2afx and H3f3a (C) in NIH3T3 cells. (D) qChIP analysis of UBTF1/2 binding to canonical and variant histone genes in NIH3T3 cells. qChIPs were performed as described in Figure 1D (n = 4; Ave ± SEM). (*) P-value < 0.05, (**) P-value < 0.01, compared to corresponding RS samples. Amplicons at Hist1h1a and major satellite repeats were used as a negative control for UBTF1/2 binding.
