Replication timing is regulated by the number of MCMs loaded at origins

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Figure 2.
Figure 2.

ARS1 has multiple MCM complexes loaded in vivo. (A) HA Western blot of protein from Mcm2-HA, Orc2-HA, Zif268-HA cells (yFS853) before and after affinity purification of an ARS1-containing TALO8 plasmid. G1 cells were arrested in α-factor; G2 cells were arrested in nocodazole. (B) Quantitation of eluate data in A: n = 7 for G1; n = 3 for G2; error bars represent SEM. (C) Western blot as in A from G1-arrested cells carrying an ARS1-containing (yFS853) or ARS316-containing (yFS855) TALO8 plasmid. (D) Quantitation of data in C: n = 7 for ARS1, n = 3 for ARS316, error bars represent SEM.

This Article

  1. Published in Advance September 10, 2015, doi: 10.1101/gr.195305.115 Genome Res. 25: 1886-1892

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