The circadian clock regulates cytoplasmic processing body formation. (A) Confocal immunofluorescence images of the P body markers, P54/RCK and GE-1/HEDLS, are shown at a representative time point when P bodies were found to be present in U2OS cells. DAPI was used as a nuclear counterstain. P body foci are indicated with arrows in the merged image. (B) Normalized RPF reads for the LSM1 gene as a function of time for both the wild-type (red) and siARNTL (gray) data. Points from both replicates are displayed, and the lines are plotted using a moving average (see Supplemental Methods for further detail). (C) A graph representing the number of cells with P bodies at the 4- and 16-h time points for both synchronized wild-type and siARNTL U2OS cells. Error bars represent standard deviations. (D) A graph representing the average number of P bodies per cell at the 4- and 16-h time points for both synchronized wild-type and siARNTL U2OS cells. Error bars represent standard deviations.
