Prepatterning of differentiation-driven nuclear lamin A/C-associated chromatin domains by GlcNAcylated histone H2B

Lamin A/C LADs form de novo on chromatin domains pre-enriched in H2BGlcNAc within 1 day of adipogenic induction. (A) Browser views of LADs and GADs during adipogenic differentiation. (B) Venn diagram analysis of overlap of de novo LADs formed on D1 with preexisting D0 GADs maintained on D1 (in megabases): Data show that 71% of de novo LAD coverage overlaps with preexisting GADs, reflecting a nonrandom association (permutation t-test P value = 9.98 × 10⁻⁶), and this de novo association concerns 50% of GADs. (C) Jaccard index of overlap of lamin A/C LADs with GADs during differentiation. (D) Genome-wide H2BGlcNAc levels positively correlate with lamin A/C levels (D1).