H2B GlcNAcylated domains (GADs) are maintained during adipogenic differentiation. (A) H2BGlcNAc ChIP-seq profiles (ChIP/input ratios, scale −0.4/+0.4), GADs identified using EDD, and RNA-seq profiles (scale 0–1000 FPKM). (B) Median GAD length and partitioning based on quantile length distribution. (C) Gene density in GADs and in the whole genome. (D) Median expression level of genes within GADs, outside GADs, and in the whole genome (All). (E) Jaccard index of overlap of small, medium, and large GADs with large (L), medium (M), and small (S) GADs between consecutive differentiation time points (x-axis). (F) Percentages of GAD coverage shared with lamin B1 LADs identified in fibroblasts (Guelen et al. 2008). (G) ChIP-qPCR analysis of lamin B1 in ASCs on D0 and D1 of differentiation, at 20 loci shown by ChIP-seq to be H2BGlcNAcylated (amplicons 1–16) or not (amplicons 17–20) (see also Supplemental Fig. 2F).
