Figure 2.
3D DNA-FISH of the SORBS2-FRG1 interaction. (A) Processed images of in situ hybridization using an FRG1 (green) and SORBS2 (red) probe. Images were processed with IMARIS for N∼30 nuclei per condition. (B) Quantification of the number of adjacent (A) and separated (S) signals. (C) Mean of the distances measured between the gravity centers of each signal. Respective mean ± SEM of the adjacent distances are shown. Independent t-test between all conditions for binary choice: P < 10 × 10−7; t-test of the mean distance between centers: P = 0.01.
