Zelda is differentially required for chromatin accessibility, transcription factor binding, and gene expression in the early Drosophila embryo

Many loci bound by Zld in wild-type embryos remain accessible in zld^M- embryos. (A) Flow chart illustrating the four classes of yw FAIRE peaks used for future analysis. (B) Average FAIRE signal for each class of FAIRE peaks in both yw and zld^M- embryos. (C) Predicted nucleosome occupancy based on DNA sequence surrounding FAIRE peaks in each of the indicated classes. Colors indicate the different FAIRE classes. Plots are centered on the FAIRE peak midpoint and oriented relative to the transcription start site of the nearest gene. (D) Pie charts showing the genomic distribution to promoters, genes, introns, and intergenic regions for each class of FAIRE peaks.