Loss of miR-58 family members does not lead to any compensation effects among other family members. (A) Small RNA sequencing comparing miRNA abundance in reads per million between different miR-58 family mutant samples and the WT. The reads are normalized to the four calibration sequences that were added in the sample in equal amounts during miRNA library preparation (marked as green stars on the plot). (B) miRNA-qRT-PCR assaying relative fold change of miR-58.1, miR-80, miR-81, and miR-82 in different miR-58 family mutant backgrounds identified no significant changes. The levels were normalized to the miRNAs stably expressed throughout the development, miR-250 and miR-52 (Kato et al. 2009), and are relative to WT. Standard deviations between three biological replicates are indicated.
