Cooperative target mRNA destabilization and translation inhibition by miR-58 microRNA family in C. elegans

Reduced relative translation efficiency of miR-58 targets in multiple, but not single, miR-58 family mutants. (A–D) Translational efficiency calculated as log₂ (fold change protein abundance/fold change mRNA abundance) for targets and 518 nontargets. P-values were calculated using a KS test comparing the fold change distributions (log₂) of targets in mir-80; mir-58.1 double and mir-80; mir-58.1; mir-81-82 quadruple mutants to the mir-58.1 single mutant (A) and of targets to nontargets (B–D). (E) Release of translational inhibition is particularly prominent in the single mir-58 mutant. Fraction of translationally repressed [log₂ fold change protein abundance (mutant/WT) > 0.05, log₂ mRNA abundance (mutant/WT) < 0.05] TargetScan-predicted targets in mir-58.1 single, mir-80; mir-58.1 double, and mir-80; mir-58.1; mir-81-82 quadruple mutants.