An extended family of novel vertebrate photopigments is widely expressed and displays a diversity of function

RNA in situ hybridization of novel opsin expression in the adult zebrafish brain collected at ZT3 (opn6a, A; opn6b, B; opn7a, C; opn7b, D; opn7c, E; opn7d, F; opn8a, G; opn8b, H; opn8c, I; and opn9, J). Two areas of the brain were analyzed in transverse section through the mesencephalon (i) and cerebellum (ii), where new opsin transcripts were located to the periventricular gray zone (PGZ), the optic tectal torus longitudinalis (TL), the corpus mamillare (CM), the granular layers of both the medial and lateral valvula cerebelli (Vam_gra and Val_gra, respectively), the granular layer of the corpus cerebellum (CCe_gra), the eminentia granularis (EG), the granular layer of the lobus caudalis cerebelli (LCa_gra), and two hypothalamic nuclei (caudal nucleus [Hc] and dorsal nucleus [Hd]).