Figure 5.
DIS3, but not EXOSC10, is involved in snoRNA processing. (A) A genome browser screenshot of the genomic region encompassing SNORD13 snoRNA with mapped RNA-seq and DIS3 PAR-CLIP reads. The uniquely mapped reads are shown for the plus strand. Note that mature snoRNA are underrepresented in the long read RNA-seq libraries. (B) Northern blot analysis of selected snoRNAs, using cell lines with DIS3 and EXOSC10 mutations. Multiple myeloma-associated mutations are represented by 766 and 780 for DIS3 G766R and DIS3 R780G, respectively (Tomecki et al. 2014).
