Efficient gene targeting by homology-directed repair in rat zygotes using TALE nucleases

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Figure 1.
Figure 1.

Assay of TALE nucleases for rat Hprt1.1, Hprt1.2, and Rosa26 loci. The frequency of TALE nuclease cleavage was determined using T7 endonuclease assay in C6 cells transfected with the indicated amount of rat Hprt1.1, Hprt1.2, or Rosa26 TALE nuclease expression vectors. Expression vectors without nucleases serve as negative controls. The expected sizes of undigested and digested fragments are indicated in italics. The efficiency of cleavage is indicated below each gel.

This Article

  1. Published in Advance July 2, 2014, doi: 10.1101/gr.171538.113 Genome Res. 24: 1371-1383

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