GAGA factor binding at HOT regions. (A) TRFs with data sets highly enriched for binding at HOT regions (Z-score >50). Only Trithorax-like/GAGA factor (Trl; shaded gray) is enriched at mid- and late-embryo stages and Kc167 cells. (B) Top five motifs enriched in HOT regions. Motif scanning was performed using FIMO (Grant et al. 2011). (C) Fraction of Trl consensus motif occurrence (green bars) and enrichment relative to random (blue bars) in COLD, WARM, and HOT regions. Trl consensus motif is as described in Adams et al. (2000). (D) Trl binding across chromosome 3L in embryos and dissected larval (L3) wing discs. (E) Scatterplots representing the Trl max ChIP-seq signal in embryo and wing disc data sets across HOT, WARM, and COLD regions; Pearson correlation (R) is indicated for each set of comparisons.
