RNA editing. (A) D. melanogaster editing events that align to the indicated genomes (black) and are used if aligned (blue). (B) Combined sequence and expression conservation for editing events. (C) Frequency of conservation index scores for MDv2-annotated edits. (Inset) Probability that CI is random (shades of black). (D) An example of a validated editing site in moleskin with a low CI. Gene model and log2 scale RNA-seq coverage in adult males with editing site are indicated (red asterisk). (E) Genome alignment of moleskin editing site (red asterisk) and flanking region. (D,E) Nucleotides are color coded (I, light blue; A, red; C, green; G, blue; T, orange). (F) Stacked bar plot of editing site base calling in D. melanogaster, D. simulans, D. yakuba, and D. kikkawai. (G) Frequencies of editing occurrence among transcripts from genes with annotated alternative transcription start sites (Alt. TSS), alternative splicing (Alt. Spliced), both alternative transcription start sites and splicing (Alt. TSS & Alt. Spliced), multiexon genes with a single annotated isoform, and single exon genes. All D. melanogaster genes (gray), those with edits in at least one other species (dark blue), and those with edits only in D. melanogaster (light blue) are shown.
