RNA splicing validation. (A) D. melanogaster MDv2 GT-AG (black) and GC-AG (green) splice junctions (recognized by U2 spliceosomes) and AT-AC splice junctions (red) (recognized by U12 spliceosomes) that align to the indicated genomes. (B) Aligned elements expressed (≥1 junction spanning read). (C) Combined sequence and expression conservation for each element type plotted against distance from D. melanogaster. (D) An example of a validated splicing event in a transcript model of the pollux gene. (Upper panel) An exon previously annotated as constitutive is annotated as an alternatively spliced cassette in MDv2 (red asterisk). (Lower panels) RNA-seq read coverage (black), and junction coverage with percent spliced in (PSI) values for the cassette exon inclusion (upper dotted lines) and exclusion (lower dotted lines) isoforms in adult females of the indicated species. Additional species also showed this splicing pattern (not shown). (E) Density plots of female/male ▵PSI values for species (and two strains in the case of D. simulans) plotted against D. melanogaster female/male ▵PSI values.
