Quantifying ChIP-seq data: a spiking method providing an internal reference for sample-to-sample normalization

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Figure 6.
Figure 6.

Spike adjustment improves the similarity of two Pol II ChIP-seq replicate experiments. (A) ECDFs of the scores of the indicated distributions. Preliminary scores were computed around the TSS (±250 bp) with the SPP software. The KS distance is shown at the bottom right of each panel. (Dark line) RPB2_90 sample; (light line) RPB2_95 sample. (B) Scatter plots showing the relation between the RPB2_90 and RPB2_95 scores before (orange dots) and after (black dots) spike adjustment. The red line corresponds to x = y.

This Article

  1. Genome Res. 24: 1157-1168

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