Figure 3.
Predicted transcription factor motifs are important for cell type-specific enhancer activity. (A) Luciferase (Luc) assays in S2 cells of wild-type (light blue) and GATA or E-box motif mutant (dark blue) sequences of three S2-specific enhancers. (B) Luciferase assays in OSCs of wild-type (light red) and Fkh motif mutant (dark red) sequences of three OSC-specific enhancers. Neg denotes a negative control sequence used for normalization (Arnold et al. 2013), and error bars indicate standard deviations of at least three independent biological replicates. Shown are P-values from unpaired t-tests.
