Figure 3.
Pol theta mediates end joining of breaks in pol eta- and pol kappa-deficient animals. (A) Fecundity of single, double, and triple knockout mutants of pol theta and TLS polymerases pol eta and pol kappa. (B) Quantification and (C) representative pictures of RAD-51 immunostainings on germlines of the indicated genotype. Scale bar, 10 µm. (D) Schematic representation of the unc-22 reporter gene and spontaneous deletions (in red) isolated from either polh-1polk-1 or polh-1polk-1polq-1 mutant animals. Three of five deletions extended beyond the borders of the unc-22 locus.
